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Selective and Sensitive Detection of Cyanide Based on the Displacement Strategy Using a Water-Soluble Fluorescent Probe.
A water-soluble fluorescent probe (C-GGH) was used for the highly sensitive and selective detection of cyanide (CN(-)) in aqueous media based on the displacement strategy. Due to the presence of the recognition unit GGH (Gly-Gly-His), the probe C-GGH can coordinate with Cu(2+) and consequently display ON-OFF type fluorescence response. Furthermore, the in situ formed nonfluorescent C-GGH-Cu(2+) complex can act as an effective OFF-ON type fluorescent probe for sensing CN(-) anion. Due to the strong binding affinity of CN(-) to Cu(2+), CN(-) can extract Cu(2+) from C-GGH-Cu(2+) complex, leading to the release of C-GGH and the recovery of fluorescent emission of the system. The probe C-GGH-Cu(2+) allowed detection of CN(-) in aqueous solution with a LOD (limit of detection) of 0.017 μmol/L which is much lower than the maximum contaminant level (1.9 μmol/L) for CN(-) in drinking water set by the WHO (World Health Organization). The probe also displayed excellent specificity for CN(-) towards other anions, including F(-), Cl(-), Br(-), I(-), SCN(-), PO4 (3-), N3 (-), NO3 (-), AcO(-), SO4 (2-), and CO3 (2-).
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