Sphingomonas fonticola sp. nov., isolated from spring water.

A bacterial strain designated TNR-2T was isolated from spring water in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TNR-2T were aerobic,Gram-stain-negative, straight rods, motile by a single polar flagellum and containing poly-β-hydroxybutyrate. The cells were covered by large capsules and formed yellow colonies.Growth occurred at 15-37 °C (optimum, 20-30 °C), with 0-1.0% NaCl (optimum, 0-0.1 %)and at pH 5.0-8.0 (optimum, pH 6.0). According to a phylogenetic tree based on 16S rRNA gene sequence analysis, strain TNR-2T belonged to the genus Sphingomonas and clustered with Sphingomonas alpina S8-3T, with which it shared the highest 16S rRNA gene sequence similarity (95.6 %). The major fatty acids (.10 %) of strain TNR-2T were C18 : 1ω7c, C17 : 1ω6c and C16 : 0. The DNA G+C content was 62.8 mol%. The major isoprenoid quinone was Q-10.The major polyamine was homospermidine. The polar lipid profile consisted ofsphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol,phosphatidylmonomethylethanolamine, phosphatidylcholine, diphosphatidylglycerol,two uncharacterized glycolipids and an uncharacterized phospholipid. Phenotypic characteristics of the novel strain differed from those of the closest related species of the genus Sphingomonas. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TNR-2T represents a novel species in the genus Sphingomonas, for which the nameS phingomonas fonticola sp. nov. is proposed. The type strain is TNR-2T (=BCRC80539T=LMG 27384T=KCTC 32258T).