Pedobacter alpinus sp. nov., isolated from a plateau lake in China.

Two Gram-staining-negative, aerobic, non-motile, red-pigment and rod-shaped bacteria strains, designated RSP19T and RSR28, were isolated from lake water and subjected to polyphasic taxonomic studies. Based on 16S rRNA gene sequence analysis, strains RSP19T and RSR28 exhibited 91.4-95.7% similarity to the type strains of existing Pedobacter species. The closest phylogenetically related species were Pedobacter daechungensis (95.7%), Pedobacter lentus (95.4%), Pedobacter glucosidilyticus (94.5%) and Pedobacter terricola (94.2%). MK-7 was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and several unidentified polar lipids. The predominant fatty acids were C16:1ω7c, iso-C15:0 and iso-C17:0 3OH. The genomic DNA G+C content were 34.3 and 33.9 mol% for strains RSP19T and RSR28. Based on the phenotypic characteristics, biochemical properties and genetic analysis, strains RSP19T and RSR28 clearly represent a novel species of the genus Pedobacter, for which the name Pedobacter alpinus was proposed. The type strain was strain RSP19T (=CGMCC 1.14040T = KCTC 42456T=NBRC 110967T ).