[Spermidine enhances osteogenic differentiation and inhibits adipogenic differentiation of bone marrow-derived mesenchymal stem cells from ovariectomized mice].

OBJECTIVE: To investigate the effects of spermidine (SPD) on the differentiation ability of bone marrow-derived mesenchymal stem cells (BMMSCs) from estrogen-deficient mouse models.

METHODS: Female C57/BL6J mice were randomly divided into sham group and ovariectomized (OVX) group. Two months after modeling, BMMSCs of sham and OVX mice were harvested and cultured, and then Western blotting was used to detect the expressions of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), peroxisome proliferator activated receptor gamma (PPARγ) and lipoprtein lipase (LPL). Mineralized nodules and lipid droplet formation ability of the BMMSCs from sham and OVX mice were evaluated and compared by alizarin red staining and oil red O staining, respectively. After BMMSCs from the OVX mice were cultured in osteogenesis-induced medium and adipogenesis-induced medium supplemented with SPD, their osteogenic and adipogenic differentiation capacities were evaluated by Western blotting and reverse transcription PCR to detect the expressions of Runx2, ALP, PPARγ and LPL, and the mineralized nodules and lipid droplet formation ability were examined by alizarin red staining and oil red O staining, respectively.

RESULTS: BMMSCs from OVX mice showed lower ability of osteogenic differentiation and higher ability of adipogenic differentiation compared to the ones from sham mice. SPD strengthened the ability of osteogenic differentiation and depressed the ability of adipogenic differentiation of BMMSCs from OVX mice compared with the BMMSCs without SPD intervention.

CONCLUSION: SPD enhances osteogenic differentiation and inhibits adipogenic differentiation of BMMSCs from OVX mice.