Characterization of a novel cyclase-like gene family involved in controlling stress tolerance in rice

Yonghua Qin, Xin Shen, Nili Wang, Xipeng Ding
Journal of Plant Physiology 2015 June 1, 181: 30-41
A novel cyclase-like gene family (CYL) encodes proteins containing cyclase domain, but their functions are largely unknown. We report the systematic identification and characterization of CYL genes in the rice genome. Five putative CYL protein sequences (OsCYL1 to 4b) were identified. These sequences and other CYL homologs were classified into four subgroups based on phylogenetic analysis. Distinct diversification of these CYL proteins exists between plants and non-plants. The CYL family has conserved exon-intron structures, and the organizations of putative motifs in plants are specifically diverse. All OsCYL genes were expressed in a wide range of tissues or organs and were responsive to at least one of the abiotic stresses and hormone treatments applied. Protein OsCYL4a is targeted to the cell membrane. The overexpression of one stress-responsive gene OsCYL4a in rice resulted in decreased tolerance to salt, drought, cold, and oxidative stress. The expression levels of some abiotic stress-responsive factors, including H2O2-accumulating negative factors DST and OsSKIPa in OsCYL4a-overexpressing plants, were reduced compared with the wild type under normal condition and drought stress. These results suggest that rice CYL family may be functionally conserved polyketide cyclase, resulting in the rapid accumulation of reactive oxygen species to decrease tolerance to abiotic stresses.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"