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Journal Article
Research Support, Non-U.S. Gov't
The phasin PhaF controls bacterial shape and size in a network-forming strain of Pseudomonas putida.
Journal of Biotechnology 2015 April 11
Pseudomonas putida N, a poly-3-hydroxyalkonate (PHA)-producing bacterium, showing ampicillin resistance, is an unusual strain. In the presence of this antibiotic, it grows as giant cells (25-50μm) forming complex networks inter-connected by micro-tubular structures. The transformation of this bacterium with a plasmid containing the gene phaF, which encodes a phasin involved in the molecular architecture of the PHA-granules, (i) restores the wild-type phenotype by reducing both bacterial size and length (coco-bacilli ranging between 0.5 and 3μm), and (ii) increases ampicillin resistance by more than 100-fold.
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