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COMPARATIVE STUDY
ENGLISH ABSTRACT
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
[Comparison and analysis of wild and cultivated Lonicerae japonicae flos from Xinmi].
OBJECTIVE: To compare and analyze the wild and cultivated Lonicerae Japonicae Flos from Xinmi.
METHODS: Macroscopic identification and microscopic identification were adopted to compare and analyze characteristics of the wild and cultivated Lonicerae Japonicae Flos from Xinmi. The content of chlorogenic acid and luteoloside were determined by the methods in the Chinese Pharmacopoeia. DNA molecular marker technology was used to identify Lonicerae Japonicae Flos of different varieties.
RESULTS: The differences in macroscopic and microscopic characteristics as well as the content of chlorogenic acid and luteoloside between them had been found. PCR amplification reaction system and procedure of Lonicerae Japonicae Flos from Xinmi were optimized. An effective primer for identification was chosen from 11 primers.
CONCLUSION: The results provide the scientific basis for quality assessment of Lonicerae Japonicae Flos from Xinmi.
METHODS: Macroscopic identification and microscopic identification were adopted to compare and analyze characteristics of the wild and cultivated Lonicerae Japonicae Flos from Xinmi. The content of chlorogenic acid and luteoloside were determined by the methods in the Chinese Pharmacopoeia. DNA molecular marker technology was used to identify Lonicerae Japonicae Flos of different varieties.
RESULTS: The differences in macroscopic and microscopic characteristics as well as the content of chlorogenic acid and luteoloside between them had been found. PCR amplification reaction system and procedure of Lonicerae Japonicae Flos from Xinmi were optimized. An effective primer for identification was chosen from 11 primers.
CONCLUSION: The results provide the scientific basis for quality assessment of Lonicerae Japonicae Flos from Xinmi.
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