[Effects of autologous plasma and gentamicin on immunophenotype and viability of cytokine-induced killer cells].

OBJECTIVE: To investigate the effects of treatment with different volumes of autologous plasma and gentamicin on immunophenotype and viability of cytokine-induced killer (CIK) cells in vitro.

METHODS: Flow cytometry (FCM) was used to analyze the effects of autologous plasma and gentamicin on different immunocyte subtypes of CIK cells. The cell count method and MTT assay were used to detect the cytotoxicity of such pretreated CIK cells on HeLa cells.

RESULTS: No significance was found in the relative amount of immunocyte subtypes CD3(+), CD56(+), CD3(+)CD4(+), CD3(+)CD8(+), CD3(+)CD56(+), CD4(+)CD25(+) between autologous plasma and control groups. Compared with control group, CD3(+), CD56(+), CD3(+)CD8(+), CD3(+)CD56(+) cells of gentamicin group were significantly down-regulated by 1.4, 1.7, 1.4 and 2.5 folds (P<0.05), respectively. Furthermore, the cytotoxicity of CIK cells in gentamicin and control groups on HeLa cells were 43.0% and 90.0%, respectively, with a statistically significant difference (P<0.05).

CONCLUSION: Autologous plasma has little effect on different subtypes of CIK cells. On the contrary, gentamicin can cause a decrease in the number of CD3(+), CD56(+), CD3(+)CD8(+), CD3(+)CD56(+) cells with some cytotoxicity.