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Journal Article
Research Support, Non-U.S. Gov't
[Effect of supplementing Qi and activating blood circulation method on protein uptake in NRK-52E cells injured by high glucose].
OBJECTIVE: To study the effect of supplementing Qi and activating blood circulation method with danshen and huangqi on protein uptake in NRK-52E cells injured by high glucose.
METHODS: The influence of combined use of huangqi and danshen on NRK-52E cells proliferation were determined by MTT method, FITC-BSA uptake were observed by fluorescence microscope and analyzed by fluorescence spectrophotometer, and megalin expression were detected by the method of immunohistochemistry.
RESULTS: Combined use of huangqi and danshen could strengthen its protection effect. The FITC-BSA uptake and expression of megalin in NRK-52E cells were decreased in high glucose environment. Combined use of huangqi and danshen could increase FITC-BSA uptake and megalin expression in high glucose-injured NRK-52E cells.
CONCLUSION: The method of supplementing Qi and activating blood circulation can protect high glucose injured NRK-52E cells and improve the function of protein uptake by increasing expression of megalin.
METHODS: The influence of combined use of huangqi and danshen on NRK-52E cells proliferation were determined by MTT method, FITC-BSA uptake were observed by fluorescence microscope and analyzed by fluorescence spectrophotometer, and megalin expression were detected by the method of immunohistochemistry.
RESULTS: Combined use of huangqi and danshen could strengthen its protection effect. The FITC-BSA uptake and expression of megalin in NRK-52E cells were decreased in high glucose environment. Combined use of huangqi and danshen could increase FITC-BSA uptake and megalin expression in high glucose-injured NRK-52E cells.
CONCLUSION: The method of supplementing Qi and activating blood circulation can protect high glucose injured NRK-52E cells and improve the function of protein uptake by increasing expression of megalin.
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