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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Sphingosine-1-phosphate suppresses cyclophosphamide induced follicle apoptosis in human fetal ovarian xenografts in nude mice.
Fertility and Sterility 2014 September
OBJECTIVE: To investigate the antiapoptosis effect of sphingosine-1-phosphate (S1P) on human fetal ovarian tissue treated by cyclophosphamide (CTX).
DESIGN: Experimental animal study.
SETTING: University center for reproductive medicine and IVF unit.
ANIMAL(S): Female immunodeficient BALB/c nude mice, 6 to 8 weeks old.
INTERVENTION(S): Human fetal ovarian tissue slowly cryopreserved then subcutaneously transplanted in immunodeficient mice.
MAIN OUTCOME MEASURE(S): Follicle survival assessed qualitatively and quantitatively using H&E staining, and cellular apoptosis of the ovarian grafts evaluated using transmission electron microscopy and DNA nick end labeling in situ (TUNEL assay).
RESULT(S): The alkylating agent CTX caused a substantial follicle loss and apoptotic DNA fragmentation in the ovarian grafts in a period of 2 weeks of transplantation. The S1P treatment significantly prevented follicular apoptosis and maintained primordial follicle population in the grafts.
CONCLUSION(S): This study shows for the first time that S1P protects primordial follicles in human ovarian grafts from a chemotherapy drug treatment via suppressing follicle apoptosis.
DESIGN: Experimental animal study.
SETTING: University center for reproductive medicine and IVF unit.
ANIMAL(S): Female immunodeficient BALB/c nude mice, 6 to 8 weeks old.
INTERVENTION(S): Human fetal ovarian tissue slowly cryopreserved then subcutaneously transplanted in immunodeficient mice.
MAIN OUTCOME MEASURE(S): Follicle survival assessed qualitatively and quantitatively using H&E staining, and cellular apoptosis of the ovarian grafts evaluated using transmission electron microscopy and DNA nick end labeling in situ (TUNEL assay).
RESULT(S): The alkylating agent CTX caused a substantial follicle loss and apoptotic DNA fragmentation in the ovarian grafts in a period of 2 weeks of transplantation. The S1P treatment significantly prevented follicular apoptosis and maintained primordial follicle population in the grafts.
CONCLUSION(S): This study shows for the first time that S1P protects primordial follicles in human ovarian grafts from a chemotherapy drug treatment via suppressing follicle apoptosis.
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