JOURNAL ARTICLE

Revisiting guidelines for integration of flow cytometry results in the WHO classification of myelodysplastic syndromes-proposal from the International/European LeukemiaNet Working Group for Flow Cytometry in MDS

A Porwit, A A van de Loosdrecht, P Bettelheim, L Eidenschink Brodersen, K Burbury, E Cremers, M G Della Porta, R Ireland, U Johansson, S Matarraz, K Ogata, A Orfao, F Preijers, K Psarra, D Subirá, P Valent, V H J van der Velden, D Wells, T M Westers, W Kern, M C Béné
Leukemia 2014, 28 (9): 1793-8
24919805
Definite progress has been made in the exploration of myelodysplastic syndromes (MDS) by flow cytometry (FCM) since the publication of the World Health Organization 2008 classification of myeloid neoplasms. An international working party initiated within the European LeukemiaNet and extended to include members from Australia, Canada, Japan, Taiwan and the United States has, through several workshops, developed and subsequently published consensus recommendations. The latter deal with preanalytical precautions, and propose small and large panels, which allow evaluating immunophenotypic anomalies and calculating myelodysplasia scores. The current paper provides guidelines that strongly recommend the integration of FCM data with other diagnostic tools in the diagnostic work-up of MDS.

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