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Preparation and evaluation of multifunctional envelope-type nano device loading siRNA.
We prepared multifunctional envelope-type nano device (MEND) loading siRNA through lipid film hydration method; the qualities of MEND were characterized by the shape, particle size, and evaluated by the encapsulation efficiency (EE), plasma stability, and transfection efficiency. The formulated MEND was found to be relatively uniform in size with a positive zeta potential. The average siRNA entrapment efficiency was 86.4%. After 48 h exposure in 50% (v/v) serum at 37°C, the siRNA in MEND had no significant degradation. When the concentration of siRNA in 24-well plates was 500 nM, the MEND had high transfection efficiency, almost 100%.
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