Development of a new rhodamine-based FRET platform and its application as a Cu2+ probe

Xiaoyu Guan, Weiying Lin, Weimin Huang
Organic & Biomolecular Chemistry 2014 June 21, 12 (23): 3944-9
We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu(2+) probe. The novel Cu(2+) probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.

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