Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment

Mamiko Asano, Mitsunobu Doi, Kimiye Baba, Masahiko Taniguchi, Makio Shibano, Satoshi Tanaka, Minoru Sakaguchi, Masanori Takaoka, Masahiko Hirata, Rika Yanagihara, Ryosuke Nakahara, Yuko Hayashi, Takako Yamaguchi, Hitoshi Matsumura, Yoshikazu Fujita
Journal of Bioscience and Bioengineering 2014, 118 (1): 98-100
Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.

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