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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Photocleavage control of nucleated DNA nanosystems--the influence of surface strand sterics.
Nanoscale 2014 Februrary 22
We use sterically inaccessible 'seed' strands, released from a surface into solution by photocleavage to initiate a nucleated DNA polymerization reaction. We demonstrate control of the quantity of 'seed' release and that hairpin steric protection of the 'seed' leads to less 'leaky' surfaces. This polymerization is a model system for surface-photocleavage initiation of sub-stoichiometric reaction cascades; these cascades should find use as a component of labs-on-chips capable of bioanalytical and DNA-computing tasks.
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