JOURNAL ARTICLE

miR-125b promotes cell proliferation by directly targeting Lin28 in glioblastoma stem cells with low expression levels of miR-125b

Yi Wan, Guan Sun, Zhimin Wang, Jun Guo, Lei Shi
Neuroreport 2014 March 26, 25 (5): 289-96
24356103
MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate protein expression by cleaving or repressing the translation of target mRNAs. Our previous studies have revealed that miR-125b is a typical overexpressed miRNA in human primary glioblastoma stem cells (GSCs). Here, we report that miR-125b was also found to be significantly underexpressed in three primary GSCs. Characterization of the effects of the underexpressed miR-125b in GSCs showed that elevated levels of miR-125b inhibited cell growth and induced cell cycle arrest in the G0/G1 phase in vitro; a reduction in miR-125b levels had the opposite effect on tumour growth and progression. Further research into the underlying mechanism demonstrated that miR-125b acts by targeting Lin28 to regulate cell growth. Lin28 is highly expressed in human embryonic stem cells and glioblastomas. We showed that the specific repression of Lin28 results in decreased GSC proliferation, and that the overexpression of Lin28 accelerates cell proliferation. Our results highlight a novel molecular interaction between miR-125b and Lin28, and miR-125b may represent a potential novel therapeutic agent for targeting the proliferation of GSCs. In view of our previous research showing that miR-125b was overexpressed in GSCs and functioned as an oncogene, here our finding was not in agreement with our previous report, which implies that the personalized treatment on GSCs may be necessary and important.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
24356103
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"