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Crystallization and preliminary crystallographic analysis of the chimeric protein LKB1-14-3-3ζ.
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 2013 December
LKB1, a tumour suppressor, plays key roles in cell polarity, cell growth and energy metabolism. 14-3-3 proteins bind to LKB1 and suppress its functions. A chimera containing 14-3-3ζ and its binding region in LKB1 was constructed and the chimeric protein LKB1-14-4-3ζ was purified and crystallized. The crystal of LKB1-14-4-3ζ diffracted to 2.9 Å resolution and belonged to space group R32, with unit-cell parameters a = b = 130.262, c = 264.960 Å. Structure determination and refinement are in progress.
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