Oleifolioside B-mediated autophagy promotes apoptosis in A549 human non-small cell lung cancer cells

Cheng-Yun Jin, Hai Yang Yu, Cheol Park, Min Ho Han, Su Hyun Hong, Kyoung-Sook Kim, Young-Choon Lee, Young-Chae Chang, Jaehun Cheong, Sung-Kwon Moon, Gi-Young Kim, Hyung-In Moon, Wun-Jae Kim, Jai-Heon Lee, Yung Hyun Choi
International Journal of Oncology 2013, 43 (6): 1943-50
The biochemical mechanisms of cell death by oleifolioside B (OB), a cycloartane-type triterpene glycoside isolated from Dendropanax morbifera Leveille, were investigated in A549 human lung carcinoma cells. Our data indicated that exposure to OB led to caspase activation and typical features of apoptosis; however, apoptotic cell death was not prevented by z-VAD-fmk, a pan-caspase inhibitor, demonstrating that OB-induced apoptosis was independent of caspase activation. Subsequently, we found that OB increased autophagy, as indicated by an increase in monodansylcadaverine fluorescent dye-labeled autophagosome formation and in the levels of the autophagic form of microtubule-associated protein 1 light chain 3 and Atg3, an autophagy-specific gene, which is associated with inhibiting phospho-nuclear factor erythroid 2-related factor 2 (Nrf2) expression. However, pretreatment with bafilomycin A1, an autophagy inhibitor, attenuated OB-induced apoptosis and dephosphorylation of Nrf2. The data suggest that OB-induced autophagy functions as a death mechanism in A549 cells and OB has potential as a novel anticancer agent capable of targeting apoptotic and autophagic cell death and the Nrf2 signaling pathway.

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