JOURNAL ARTICLE

Rhodamine-based "turn-on" fluorescent probe with high selectivity for Fe(2+) imaging in living cells

Gui-Ge Hou, Chun-Hua Wang, Ju-Feng Sun, Mei-Zi Yang, Dong Lin, Hong-Juan Li
Biochemical and Biophysical Research Communications 2013 October 4, 439 (4): 459-63
24025683
A rhodamine-based "turn-on" fluorescent probe 1 was synthesized with high yield. The recognizing behavior displays high selectivity of 1 toward Fe(2+) with a 2:1 complex, and 1 exhibits a stable response for Fe(2+) over a concentration range from 2 μM to 24 μM. Most importantly, probe is hardly interfered by other transition metal ions. Their fluorescent enhancement is observed in the presence of Fe(2+) because of the ring-open interactions of spirocyclic. All measurements are made in PBS buffer environments simulating biological conditions to make them suitable candidates for fluorescent labeling of biological systems. Confocal laser scanning microscopy experiments have proven that probe can be used to monitor Fe(2+) in living cells.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
24025683
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"