Random-access optical-resolution photoacoustic microscopy using a digital micromirror device

Jinyang Liang, Yong Zhou, Amy W Winkler, Lidai Wang, Konstantin I Maslov, Chiye Li, Lihong V Wang
Optics Letters 2013 August 1, 38 (15): 2683-6
We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.

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