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Clostridium acidurici electron-bifurcating formate dehydrogenase.

Shuning Wang, Haiyan Huang, Jörg Kahnt, Rudolf K Thauer
Applied and Environmental Microbiology 2013 October
Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.

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