Short-term and long-term effects of excessive consumption of saturated fats and/or sucrose on metabolic variables in Sprague Dawley rats: a pilot study.

BACKGROUND: Feeding high-fat and/or high-sugar diets to rats leads to a change in markers of metabolic syndrome. However, types and amounts of fat and sugar as well as the length of the experiment for establishing diet-induced metabolic syndrome in the Sprague Dawley (SD) rat model remain uncertain. This study was designed to investigate the effects in SD rats of consuming excess lard, sucrose or a combination of lard and sucrose for a short (4 week) or long (8 week) period of time.

RESULTS: Consumption of the high-fat high-sugar (HFHS) diet significantly increased weight gain and abdominal fat weights (P < 0.05), and the rats also began to develop signs of impaired glucose tolerance and had increased fasting blood lipids glucose and insulin concentrations. The high-fat (HF) diet mainly affected weight gain and fat deposition, whereas the high-sugar (HS) diet induced glucose intolerance but not the obesity-related parameters. Control rats showed a tendency towards insulin resistance and glucose intolerance when fed for a long-term period.

CONCLUSION: The lard plus sucrose-based HFHS diet is the most efficient one for inducing signs of metabolic syndrome, and SD rats fed this diet for 8 weeks successfully develop obesity and insulin resistance, which can be used as a model for metabolic syndrome research.