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Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A.

Jing Zhang, Satoshi Yamaguchi, Hidehiko Hirakawa, Teruyuki Nagamune
Journal of Bioscience and Bioengineering 2013 September
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.

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