Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds.

A Gram-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, designated WR-R1Y(T), was isolated from soil at a field of reeds in South Korea. Strain WR-R1Y(T) grew optimally at 30 °C, at pH 7.0-7.5 and in the absence of NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain WR-R1Y(T) fell within the clade comprising Mucilaginibacter species, coherently clustering with the type strain of Mucilaginibacter composti, with which it exhibited the highest 16S rRNA gene sequence similarity value of 97.6 %. Sequence similarities to the type strains of the other Mucilaginibacter species and the other species used in the phylogenetic analysis were 93.1-96.9 % and <91.1 %, respectively. Strain WR-R1Y(T) contained MK-7 as the predominant menaquinone and iso-C15:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified aminophospholipid. The DNA G+C content of strain WR-R1Y(T) was 43.1 mol% and its mean DNA-DNA relatedness value with M. composti KACC 14956(T) was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain WR-R1Y(T) is separate from other Mucilaginibacter species. On the basis of the data presented, strain WR-R1Y(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter calamicampi sp. nov. is proposed. The type strain is WR-R1Y(T) (= KCTC 32214(T) = CCUG 63418(T)).