miR-154 inhibits prostate cancer cell proliferation by targeting CCND2

Chen Zhu, Pengfei Shao, Meiling Bao, Pu Li, Hai Zhou, Hongzhou Cai, Qiang Cao, Liangjun Tao, Xiaoxin Meng, Xiaobing Ju, Chao Qin, Jie Li, Changjun Yin
Urologic Oncology 2014, 32 (1): 31.e9-16

BACKGROUND: Research has shown reduced expression levels of miR-154 in prostate cancer (CaP). However, the function and molecular mechanisms of miR-154 in this cancer type remains unknown.

OBJECTIVE: The aims of this study were to examine the functional significance of miR-154 in CaP cells and to identify the novel molecular targets regulated by miR-154.

MATERIALS AND METHODS: miR-154 expression significantly decreased in primary CaP samples compared with nonmalignant samples measured by quantitative reverse transcription polymerase chain reaction. Restoration of miR-154 lowered the potential of CaP cell lines to grow and proliferate in vitro evaluated by CCK-8 assay, colony formation assay, and flow cytometry. miR-154 down-regulated the expression of CCND2 by binding to its 3'-untranslated region by luciferase reporter assay.

CONCLUSIONS: miR-154 plays a prominent role in CaP proliferation by suppressing CCND2, and it may provide a new approach to the treatment of CaP.

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