JOURNAL ARTICLE

In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy

Wei Shi, Peng Shao, Parsin Hajireza, Alexander Forbrich, Roger J Zemp
Journal of Biomedical Optics 2013, 18 (2): 26001
23377002
The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.

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