Scaffold effects on osteogenic differentiation of equine mesenchymal stem cells: an in vitro comparative study

Rodolfo Nino-Fong, Laurie A McDuffee, Blanca P Esparza Gonzalez, M Ramesh Kumar, Erika F Merschrod S, Kristin M Poduska
Macromolecular Bioscience 2013, 13 (3): 348-55
The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen-based scaffolds or with fibrin glue. The results indicate that bone marrow cells are the best source of MSCs for osteogenic differentiation, and that an electrochemically aggregated collagen gives the highest cell viability and best osteogenic differentiation among the four kinds of scaffolds studied.

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