We have located links that may give you full text access.
Journal Article
Research Support, Non-U.S. Gov't
Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells.
Veterinary Immunology and Immunopathology 2013 January 16
The endometrium is easily contaminated with bacteria and the endometrial epithelial cells (EECs) play an important role in defence against invading pathogens which recognized pathogen-associated molecular patterns (PAMPs) via pattern recognition receptors (PRRs). Toll-like receptor 4 (TLR4) can recognize lipopolysaccharide (LPS) from Gram-negative bacteria and initiates innate immune responses. In this study, we stimulated bovine EECs with LPS from Escherichia coli (E. coli). The expression of TLR4 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The expression of downstream TLR4 signaling molecules was detected by qRT-PCR. The results showed that TLR4 and downstream adaptor molecules, transcription factors and cytokines were up-regulated when bovine EECs were stimulated with LPS. Furthermore, the expression of TOLLIP and β-defensin 5 were up-regulated when cells were stimulated with LPS. The results demonstrated that both MyD88 dependent and independent pathways in TLR4 were activated by LPS in bovine EECs. Bovine EECs have the immune repertoires required in defending against E. coli and play an important role in innate immune defence of the bovine endometrium.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app