JOURNAL ARTICLE

The effects of bilateral common carotid artery occlusion on expression of peripherin and choline acetyltransferase activity in C57BL/6 mice

Xingjuan Zhao, Huifang Zhang, Jian Guo, Yan Zhang
Brain Research 2013 January 23, 1491: 167-75
23165118
Peripherin is a 57-kDa type III neuronal intermediate filament protein that appears to play a role in neurite elongation during development and axonal regeneration. Its role in the pathogenesis of cognitive deficits caused by cerebral ischemia is unknown. The purpose of this study was to investigate the location and level of peripherin expression in the central nervous system in response to transient global cerebral ischemia, and the resultant effect of peripherin expression on the cholinergic neurons and Choline acetyltransferase (ChAT) activity in this mouse model of ischemia. Transient global cerebral ischemia was induced in C57BL/6 mice by 20-min bilateral common carotid artery occlusion (BCCAO) with microclips. The resulting impairment of spatial learning and memory was investigated by Morris water maze testing. Peripherin expression was evaluated by immunostaining and Western Blot assay of brain sections. Peripherin expression increased in neurons of the cortex, hippocampus, and thalamus after BCCAO. By double immunofluorescence staining, neurons showed a cytoplasmic co-localization of peripherin and MAP-2, but not of peripherin and GFAP. ChAT activity was determined spectrophotometrically using the assay kit. There was significantly decreased ChAT activity in the cerebral cortex, hippocampus and thalamus in mice of the BCCAO group (p<0.05), compared with the sham group. After BCCAO, peripherin overexpression was significantly correlated with reduction in ChAT activity (r=-0.929; p<0.01), spatial learning and memory were impaired, and peripherin expression was induced in neurons but not astrocytes. Thus, peripherin appears to be a participant in learning and memory impairment in mice.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
23165118
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"