Increased expression of a gene coding for NAD:glyceraldehyde-3-phosphate dehydrogenase during the transition from C3 photosynthesis to crassulacean acid metabolism in Mesembryanthemum crystallinum

J A Ostrem, D M Vernon, H J Bohnert
Journal of Biological Chemistry 1990 February 25, 265 (6): 3497-502
We utilized differential plaque hybridization to identify three cDNA clones for transcripts which increase in abundance during the salinity-induced transition from C3 photosynthesis to crassulacean acid metabolism (CAM) in Mesembryanthemum crystallinum. Although there are differences in the abundance of these transcripts in unstressed tissue, steady-state levels of all three increased within 30 h following irrigation with 0.5 M NaCl. One cDNA encodes the cytosolic form of glyceraldehyde-3-phosphate dehydrogenase (D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating) (NAD-GAPDH], an enzyme involved in the production of phosphoenolpyruvate for CO2 fixation at night and the conversion of pyruvate to storage carbohydrate during the day. Coding region and 3'-noncoding sequence probes were used to examine the expression of NAD-GAPDH transcripts in leaf and root tissue. We show that the gene encoding the NAD-GAPDH cDNA is expressed in both leaf and root tissue during C3 photosynthesis and CAM. NAD-GAPDH transcript levels increase rapidly in leaf (but not in root) tissue during the transition to CAM. Our data indicate that the predominant NAD-GAPDH transcript expressed during C3 photosynthesis and CAM is encoded by a single gene in M crystallinum. These results imply that the transition to CAM in some cases involves an upward readjustment in the level of a gene product expressed during C3 photosynthesis, rather than the expression of a CAM-specific isoform with unique regulatory or kinetic properties.

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