JOURNAL ARTICLE

Gemmobacter tilapiae sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater pond

Shih-Yi Sheu, Der-Shyan Sheu, Fu-Sian Sheu, Wen-Ming Chen
International Journal of Systematic and Evolutionary Microbiology 2013, 63: 1550-6
22888190
A novel bacterium, designated strain Ruye-53(T), was isolated from a freshwater pond used to rear tilapiine cichlid fish in Taiwan. The taxonomic position of the novel strain was determined using a polyphasic approach. Strain Ruye-53(T) was Gram-reaction-negative, aerobic, cream-white coloured, rod-shaped, non-motile and poly-β-hydroxybutyrate-accumulating. Growth occurred at 10-37 °C (optimum between 20 and 25 °C), at pH 6.0-9.0 (optimum between pH 8.0 and pH 9.0) and with 0-1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-53(T) belonged to the genus Gemmobacter and that its most closely related neighbour was Gemmobacter aquatilis DSM 3857(T) (97.6 % sequence similarity). The novel strain's predominant fatty acid was C18 : 1ω7c, its major respiratory quinone was Q-10 and its genomic DNA G+C content was 61.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one uncharacterized aminolipid and four uncharacterized phospholipids. The DNA-DNA relatedness value between strain Ruye-53(T) and Gemmobacter aquatilis DSM 3857(T) was about 45.5 % (48.2±0.4 % in the reciprocal experiment). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ruye-53(T) represents a novel species in the genus Gemmobacter, for which the name Gemmobacter tilapiae sp. nov. is proposed. The type strain is Ruye-53(T) ( = BCRC 80261(T)  = KCTC 23310(T)).

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
22888190
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"