Development and validation of a UPLC-MS/MS method for studying the degradation kinetics of ofloxacin and trimethoprim during the application of solar Fenton process in secondary treated sewage.

In this work, a sensitive and highly selective method was developed and validated to study the degradation of two antibiotic compounds (ofloxacin (OFX) and trimethoprim (TMP)), spiked in secondary treated domestic effluents, by the solar Fenton process. Three different chromatographic columns were tested on a ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) instrument working in the electrospray ionization (ESI) mode with twelve combinations of eluting solvents. Samples were enriched prior to the analysis by solid phase extraction using the hydrophilic-lipophilic balanced (HLB) reversed phase polymeric sorbent. The method was optimized and showed very good performance characteristics and was successfully applied to study the degradation kinetics of the selected antibiotics during the solar Fenton process applied. The degradation was found to follow a pseudo first-order kinetics for both compounds at initial concentration of 100 μg L(-1) with k = 0.0345 min(-1) for OFX and k = 0.0768 min(-1) for TMP, whereas the complete removal was achieved after 120 min of treatment for both compounds.