JOURNAL ARTICLE

A highly sensitive fluorescent probe for HClO and its application in live cell imaging

Qing-Ping Zuo, Zuo-Jun Li, Yong-Hua Hu, Bin Li, Li-Hua Huang, Chun-Jiang Wang, Shi-Kun Liu, Hai-Qiang Liao
Journal of Fluorescence 2012, 22 (5): 1201-7
22802149
A new rhodamine-based probe 1 was designed and synthesized as a new fluorescent molecular probe for HClO in PBS buffer at physiological condition. The free probe 1 almost nonfluorescence, however, a drastic enhancement of fluorescence intensity was observed in the presence of HClO. The new probe 1 exhibits good sensitivity and selectivity for HClO over other reactive oxygen and/or nitrogen species in PBS buffer, and the probe was successfully applied to image endogeneous HClO in the living cells.

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