[Influence of antistaphylococcus antiobiotic batumin on microorganism biofilm formation].

AIM: Study the influence of batumin on microorganism biofilm formation.

MATERIALS AND METHODS: Experimental data on the antimicrobial effect of batumin on microorganisms and biofilm formation (BFF) was obtained by studying 80 strains of bacteria and fungi isolated from microbial biocenosis of the nose of staphylococcus carriers and patients during examination for intestine dysbiosis. 80% pure batumin was used in the experiments. Antimicrobial activity of batumin was studied by serial dilutions method (CLSI, 2005), BFF--by photometry method (O'Toole G., 2000). The results were statistically treated by non-parametric method by using Mann-Whitney criterion.

RESULTS: Minimal inhibitory concentration (MIC) of batumin varied from 0.25 mcg/ml to 64 mcg/ml depending on the species of the studied microorganisms. The most sensitive to batumin strains were Staphylococcus aureus when compared with escherichia and klebsiella. Batumin had no antimicrobial effect on the studied Candida albicans. Inhibitory effect of batumin against BFF of staphylococci, klebsiella and fungi that have an initial level of this property above 2.5 units was established. While in strains that have the initial BFF level of 2.5 units or less, batumin stimulated biofilm formation. Such a dependence was not detected in the studied escherichia coli cultures: batumin stimulated BFF of Escherichia in 80 - 90% of cases.

CONCLUSION: The data obtained uncover one of the possible mechanisms of microsymbiocenosis formation in the human associative symbiosis and open the perspectives for further studies of batumin not only as an antimicrobial preparation but also a substance possessing anti-persistence effect against pathogens.