Derxia lacustris sp. nov., a nitrogen-fixing bacterium isolated from a freshwater lake

Wen-Ming Chen, Wei-Cheng Huang, Shih-Yi Sheu
International Journal of Systematic and Evolutionary Microbiology 2013, 63 (Pt 3): 965-70
A novel nitrogen-fixing strain, designated HL-12(T), was isolated from a freshwater lake in Taiwan. Cells of strain HL-12(T) were aerobic, Gram-negative, motile rods that were surrounded by a thick capsule, contained poly-β-hydroxybutyrate granules, and formed light-yellow to brownish-red colonies. Growth occurred at 15-40 °C (optimum 25-35 °C), at pH 6.0-7.0 (optimum pH 6.0) and with 0-4 % NaCl (optimum 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HL-12(T) belonged to the genus Derxia and exhibited 99.1 and 98.8 % 16S rRNA gene sequence similarity, respectively, with Derxia gummosa IAM 14990 and D. gummosa IAM 13946(T). The major fatty acids (>10 %) of strain HL-12(T) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The cellular hydroxy fatty acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 72.0 mol%. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several unknown aminophospholipids and phospholipids. DNA-DNA relatedness between strain HL-12(T) and LMG 3975 and LMG 3977(T) was <70 %. On the basis of the genotypic and phenotypic data, strain HL-12(T) represents a novel species in the genus Derxia, for which the name Derxia lacustris sp. nov. is proposed. The type strain is HL-12(T) ( = BCRC 80208(T)  = KCTC 23311(T)).

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"