JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
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A rhodamine chromene-based turn-on fluorescence probe for selectively imaging Cu²⁺ in living cell.

We describe the development of a rhodamine chromene-based turn-on fluorescence probe to monitor the intracellular Cu(2+) level in living cells. The new fluorescent probe with a chlorine group in chromene moiety exhibits good membrane-permeable property than previous reported because the predicted lipophilicity of present probe 4 is stronger than that of methoxyl substituted probe in our previous work (CLogP of 4: 8.313, CLogP of methoxyl substituted probe: 7.706), and a fluorescence response toward Cu(2+) under physiological conditions with high sensitivity and selectivity, and facilitates naked-eye detection of Cu(2+). The fluorescence intensity was remarkably increased upon the addition of Cu(2+) within 1 or 2 min, while the other sixteen metal ions caused no significant effect.

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