JOURNAL ARTICLE

Minimally modified LDL upregulates endothelin type B receptors in rat basilar artery

Jie Li, Yong-Xiao Cao, Yong Liu, Cang-Bao Xu
Microvascular Research 2012, 83 (2): 178-84
22198335
Minimally modified low density lipoprotein (mmLDL) is a well-known risk factor for cerebral vascular diseases and upregulation of endothelin type B (ET(B)) receptors plays key roles in the pathogenesis. The present study was designed to examine if mmLDL upregulated endothelin ET(B) receptors in basilar artery and its possible intracellular signaling molecular mechanisms. Rat basilar arteries were cultured for 24h in the presence of mmLDL with specific inhibitors. The artery contractile responses and receptor expressions of mRNA and protein were investigated using myograph system, real-time PCR and Western blot techniques, respectively. Results showed that ET(B) receptor agonist, sarafotoxin 6c induced a weak contraction in fresh basilar artery segments. After organ culture the contraction curve mediated by ET(B) receptor was shifted towards the left with an increased E(max) of 88 ± 6%. The mmLDL 10 μg/mL further shifted the concentration-contractile curves towards the left with an increased E(max) of 116 ± 12%. The organ culture significantly increased ET(B) receptor mRNA and protein levels from fresh arteries, which was further enhanced by mmLDL. The staurosporine (PKC inhibitor), both SB386023 and U0126 (extracellular signal related kinases 1 and 2 inhibitor), and wedelolactone (NF-κB inhibitor) almost totally abolished organ culture-increased and mmLDL-increased contraction and expressions of endothelin ET(B) receptor. SP600125 (C-jun terminal kinase inhibitor) and SB203580 (p38 inhibitor) attenuated both organ cultured-induced and mmLDL-induced upregulation of endothelin ET(B) receptors. In conclusion, mmLDL upregulates ET(B) receptors of cerebral basilar artery via the PKC, MAPK and NF-κB signal pathways.

Full Text Links

Find Full Text Links for this Article

Discussion

You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read
22198335
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"