Enhanced vascularization by controlled release of platelet-rich plasma impregnated in biodegradable gelatin hydrogel

Jiro Kurita, Masaaki Miyamoto, Yosuke Ishii, Junya Aoyama, Gen Takagi, Zenya Naito, Yasuhiko Tabata, Masami Ochi, Kazuo Shimizu
Annals of Thoracic Surgery 2011, 92 (3): 837-44; discussion 844

BACKGROUND: Platelet-rich plasma (PRP) contains numerous growth factors that have angiogenic activities. However, the PRP-induced angiogenesis is limited by the short half-life period of growth factors. A new drug delivery system of biodegradable gelatin hydrogel was designed to achieve the controlled release of growth factors in PRP. The purpose of this study is to demonstrate the therapeutic efficacy of slow-release of PRP in the inducing of angiogenesis for critical ischemia.

METHODS: The PRP was prepared from the whole blood of inbred rats. Thirty-two rats underwent excision of the left femoral artery and its branches to create critical limb ischemia. The rats were randomized into four groups (n=8 each): no treatment (control), intramuscular injection of platelet-poor plasma (PPP), PRP only, or a combination of PRP and gelatin hydrogel (PRP+Gel). Four weeks after the treatment, angiogenesis was evaluated by laser doppler, microangiogram, and immunohistology.

RESULTS: The resultant number of platelets for PRP was higher than that of PPP (p<0.01). The concentrations of vascular endothelial growth factor, transforming growth factor-β1, and platelet-derived growth factor-BB were significantly higher in PRP animals than in PPP (p<0.01). Although the PRP group improved tissue blood flow (82.7%±6.2%) compared with the control group or PPP group (69.6±12.2 or 72.2±11.8%, p<0.05), the improvement of blood flow in the PRP+Gel group was significantly better (95.1%±8.0%, p<0.05) than in the PRP group. Angiographic score in the PRP+Gel group was significantly higher than that in the control, PPP, and PRP groups (8.6±2.1 versus 3.8±0.8, 3.7±0.6, and 5.6±1.5, respectively; p<0.01). Capillary density also increased immunohistologically in the PRP+Gel group when compared with the control, PPP, and PRP groups (p<0.01).

CONCLUSIONS: A controlled release system of PRP was effective in inducing angiogenesis for critical ischemia. The biodegradable gelatin hydrogel incorporating PRP as applicable could possibly be used to treat for patients with ischemic cardiomyopathy.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"