Inverse association between miR-194 expression and tumor invasion in gastric cancer

Yongxi Song, Feng Zhao, Zhenning Wang, Zhuangkai Liu, Yeunpo Chiang, Yingying Xu, Peng Gao, Huimian Xu
Annals of Surgical Oncology 2012, 19: S509-17

BACKGROUND: MiR-194 has been shown to be specifically expressed in the human gastrointestinal tract and may play an antimetastatic role in primary liver cancer cells. However, the role of miR-194 in gastric cancer is still unclear.

METHODS: Total RNA was extracted from tissues of 119 patients with gastric cancer and three gastric cancer cell lines (SGC-7901, MGC-803, and BGC-823). Expression levels of miR-194 were determined by real-time polymerase chain reaction (PCR). Moreover, a MTT proliferation assay and transwell cell invasion assay were performed to study the effect of miR-194 on SGC-7901 cell proliferation and invasion. Finally, we used real-time PCR and western blot to verify which gene was the target of miR-194 in gastric cancer.

RESULTS: Though there was no significant difference between cancerous and matching noncancerous tissues, we found patients with lower expression of miR-194 tended to have larger tumor size (P = 0.002) and more advanced pT stage (P = 0.028) in gastric cancer. Moreover, the expression of miR-194 was significantly lower in Borrmann IV type gastric cancer than in Borrmann I, II, and III types (P = 0.019). Furthermore, an in vitro invasion assay indicated that the penetrated cell intensity after miR-194 mimics transfection was significantly lower than the control. However, overexpression of miR-194 had little effect on the SGC-7901 cell cycle and proliferation. The results of real-time PCR and western blot highlighted that miR-194 interacted with N-cadherin and negatively regulated its expression at the translational level.

CONCLUSION: These findings imply that miR-194 might play an important role in gastric cancer invasion and progression.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"