In vitro elution and antibacterial activity of clindamycin, amikacin, and vancomycin from R-gel polymer

Leslie A Thomas, Tatiana Bizikova, Anne C Minihan
Veterinary Surgery 2011, 40 (6): 774-80

OBJECTIVE: To characterize the in vitro elution and bioactivity of 2 formulations of antibiotics in a novel, dissolvable, cross-linked dextran polymer matrix: Formulation 1-amikacin and clindamycin (AC); Formulation 2-amikacin, clindamycin, and vancomycin (ACV).

STUDY DESIGN: Prospective, in vitro, experimental study.

METHODS: Aliquots of the antibiotic impregnated polymer were incubated in PBS buffer for 10 days. PBS was changed every 24 hours and concentrations of the antibiotics eluted into saline were quantified. Antimicrobial activity of the eluent from each sampling period was tested for growth inhibition of Staphylococcus aureus.

RESULTS: Both formulations of R-gel(™) had a rapid initial release of antibiotics within the first 24 hours and then the concentrations decreased gradually over 10 days. The concentration of amikacin, clindamycin, and vancomycin remained above the breakpoint minimum inhibitory concentration of each drug for a minimum of 9 days. No significant difference (P=.9938, P=.9843) was present in the elution pattern or total amount of antibiotic eluted from clindamycin or amikacin, respectively. Eluent from both groups demonstrated bioactivity against S. aureus for the entire 10-day study period.

CONCLUSIONS: Amikacin and clindamycin together, or in combination with vancomycin, elute from R-gel(™) effectively and at gradually decreasing concentrations for at least 10 days. The antibiotics maintained their bioactivity following polymerization and elution from the R-gel(™) .

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"