Homologous overexpression of omcZ, a gene for an outer surface c-type cytochrome of Geobacter sulfurreducens by single-step gene replacement

Insoon Park, Byoung-Chan Kim
Biotechnology Letters 2011, 33 (10): 2043-8
The electron transfer pathway of Geobacter sulfurreducens has been intensively studied because of its ability of electron transfer to extracellular electron acceptors, such as Fe(III) and on electrode. However, the absence of overexpression system of G. sulfurreducens is one of the main obstacles for studying the physiology of G. sulfurreducens. OmcZ, an outer membrane-related c-type cytochrome of G. sulfurrducens, was homologously overexpressed via genomic integration. Quantitative RT-PCR analysis showed that the omcZ transcript in the knock-in strain was sixfold more abundant than in the wild type. Notably, omcZ expression appears to downregulate the expression level of OmcS, another outer membrane-related c-type cytochrome of G. sulfurreducens, based on the comparisons of total protein and transcript levels. This is the first report of the successful genetic overexpression system for studying functional genomics of G. sulfurreducens.

Full Text Links

Find Full Text Links for this Article


You are not logged in. Sign Up or Log In to join the discussion.

Related Papers

Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"