Connective tissue growth factor and tissue inhibitor of matrix metalloproteinase-2 in patients with exfoliative glaucoma.

PURPOSE: To investigate the aqueous humor levels of connective tissue growth factor (CTGF), matrix metalloprotinease-2 (MMP-2), and tissue inhibitor of matrix metalloprotinease-2 (TIMP-2) in human eyes with exfoliative glaucoma, primary open-angle glaucoma, and senile cataract patients.

PATIENTS AND METHODS: This was a prospective, comparative study. Sixty patients with glaucomas and 25 patients with senile cataract of matched age and gender were enrolled in this study. Patients were classified into three groups: group I comprised 30 patients with exfoliative glaucoma (XFG), group II comprised 30 patients with primary open-angle glaucoma (POAG), and group III comprised 25 patients with senile cataract (controls). Aqueous humor samples were obtained by paracentesis at the time of elective surgery for glaucomatous and cataractous patients. CTGF, MMP-2, and TIMP-2 were measured in aqueous humor by specific enzyme linked immunosorbent assay (ELISA) kits, and total aqueous humor protein content was assessed by the Lowry method.

RESULTS: There were significant increases in aqueous humor levels of CTGF and TIMP-2 in XFG patients compared to the corresponding values of POAG patients or controls. The MMP-2 aqueous humor level was significantly increased in the XFG patients when compared with controls (P < 0.001). Moreover, the total protein level in the aqueous humor of eyes of the XFG patients was significantly higher than in POAG patients or controls (P < 0.001). A positive correlation was found between CTGF and MMP-2 in aqueous humor samples of XFG patients (P < 0.001).

CONCLUSION: Increased levels of aqueous humor of CTGF and TIMP-2 may promote the abnormal extracellular matrix accumulation and may be involved in the pathogenesis of XFG.