Synthesis, crystal structure and living cell imaging of a Cu(2+)-specific molecular probe

Wei-Yong Liu, Hai-Ying Li, Bao-Xiang Zhao, Jun-Ying Miao
Organic & Biomolecular Chemistry 2011 July 7, 9 (13): 4802-5
We report the development of a rhodamine chromene-based fluorescence probe to monitor the intracellular Cu(2+) level in living cells. The new fluorescent probe exhibits a fluorescence response towards Cu(2+) under physiological conditions with high sensitivity and selectivity, and facilitates the naked-eye detection of Cu(2+). The fluorescence intensity was significantly increased by about 40-fold with 10 equiv. of added Cu(2+).

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