JOURNAL ARTICLE

Extract of the dried heartwood of Caesalpinia sappan L. attenuates collagen-induced arthritis

You-Zhi Wang, Shi-Qin Sun, Ya-Bin Zhou
Journal of Ethnopharmacology 2011 June 14, 136 (1): 271-8
21557995

AIM OF THIS STUDY: To confirm the anti-arthritic effect and explore the potential mechanism of the dried heartwood of Caesalpinia sappan L. (HCS) on collagen-induced arthritis (CIA) in rats, an animal model of rheumatoid arthritis.

MATERIALS AND METHODS: CIA was induced in male Wistar rats by intradermal injection of bovine collagen-II in Freund's incomplete adjuvant (IFA). The rats in the onset of arthritis were treated daily with oral administration of an ethanol extract from HCS (EHCS) at different doses (1.2, 2.4 and 3.6g/kg) or olive oil-vehicle for 10 days. Paw swelling, arthritis index, radiographic and histopathologic changes were evaluated to confirm the anti-arthritic effect of EHCS on CIA in rats. Levels of proinflammatory cytokines interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) as well as prostaglandin E2 (PGE2) in blood and expression of cyclooxygenase-2 (COX-2) and transcription factor NF-κB p65 in paw cartilage were detected to further study the molecular mechanism of the anti-arthritic effects of EHCS on CIA in rats. In addition, the adverse effects of EHCS on liver and kidney of rats were also evaluated.

RESULTS: The results showed that the EHCS markedly attenuated collagen-induced arthritis and reduced the levels of IL-1β, IL-6, TNF-α and PGE2 in serum and the expression of COX-2 and transcription factor NF-κB p65 in paw cartilage of CIA rats. EHCS (3.6g/kg) induced slight hepatotoxicity and body weight loss.

CONCLUSION: These results indicate that EHCS significantly attenuates CIA in rats by decreasing the levels of IL-1β, IL-6, TNF-α and PGE2 in serum and the expression of COX-2 and transcription factor NF-κB in paw cartilage.

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