We have located links that may give you full text access.
JOURNAL ARTICLE
RESEARCH SUPPORT, N.I.H., EXTRAMURAL
RESEARCH SUPPORT, NON-U.S. GOV'T
Cardiovascular magnetic resonance imaging in delivering and evaluating the efficacy of hepatocyte growth factor gene in chronic infarct scar.
BACKGROUND: In an open-chest model of acute infarct, epicardial delivery of hepatocyte growth factor (pCK-HGF-X7) gene improved left ventricle (LV) function. This study was designed to test (a) the efficacy of HGF gene in infarct scar delivered under magnetic resonance (MR) guidance and (b) the potential of multiple MR sequences in assessing the effects of pCK-HGF-X7 (treatment) and pCK-LacZ (control) genes on myocardial structure and function.
MATERIALS AND METHODS: Swine (six per group) were subjected to myocardial infarct, under X-ray fluoroscopy, and developed LV remodeling at 5 weeks. Multiple clinical magnetic resonance (MR) imaging sequences were performed before delivery of gene (at 5 weeks after infarction) and 5 weeks after delivery of gene. Under MR guidance, the active endovascular catheter was introduced into LV to transendocardially deliver 3.96 × 10(11) viral copies of pCK-HGF-X7 or pCK-LacZ in the border and core of the infarct scar. Histological evaluation of the infarct scar was performed 5 weeks after delivery of gene.
RESULTS: At 5 weeks after infarction, there was no significant difference in measured cardiovascular MR parameters between the groups. The pCK-HGF-X7 gene caused significant improvement in the following parameters (P<.05 for these parameters): three-dimensional (3D) strain (radial, circumferential, and longitudinal) and perfusion (maximum upslope, peak signal intensity, and time to peak) compared with control pCK-LacZ at 5 weeks after delivery of the genes. The ejection fraction was higher in pCK-HGF-X7-treated (43 ± 1%) than in pCK-LacZ control (37 ± 1%, P<.05) animals. These changes are associated with a decrease in infarct scar size (11.3 ± 2.0% in pCK-LacZ control and 6.7 ± 1.3% in pCK-HGF-X7-treated animals, P<.01) and infarct transmurality in four out of five infarct scar segments (P<.05) on delayed contrast-enhanced MR imaging. Microscopic study confirmed the increase in capillary (P<.05) and arteriole (P<.05) density of infarct scar in pCK-HGF-X7-treated animals compared with pCK-LacZ control animals.
CONCLUSIONS: Hepatocyte growth factor gene (pCK-HGF-X7) delivered under MR guidance into infarct scar ameliorated global function and 3D strain, increased regional perfusion and infarct resorption, and enhanced angiogenesis/arteriogenesis. This feasibility study provides novel approach and analysis methods and instrumentation for delivering and evaluating new locally delivered therapies.
MATERIALS AND METHODS: Swine (six per group) were subjected to myocardial infarct, under X-ray fluoroscopy, and developed LV remodeling at 5 weeks. Multiple clinical magnetic resonance (MR) imaging sequences were performed before delivery of gene (at 5 weeks after infarction) and 5 weeks after delivery of gene. Under MR guidance, the active endovascular catheter was introduced into LV to transendocardially deliver 3.96 × 10(11) viral copies of pCK-HGF-X7 or pCK-LacZ in the border and core of the infarct scar. Histological evaluation of the infarct scar was performed 5 weeks after delivery of gene.
RESULTS: At 5 weeks after infarction, there was no significant difference in measured cardiovascular MR parameters between the groups. The pCK-HGF-X7 gene caused significant improvement in the following parameters (P<.05 for these parameters): three-dimensional (3D) strain (radial, circumferential, and longitudinal) and perfusion (maximum upslope, peak signal intensity, and time to peak) compared with control pCK-LacZ at 5 weeks after delivery of the genes. The ejection fraction was higher in pCK-HGF-X7-treated (43 ± 1%) than in pCK-LacZ control (37 ± 1%, P<.05) animals. These changes are associated with a decrease in infarct scar size (11.3 ± 2.0% in pCK-LacZ control and 6.7 ± 1.3% in pCK-HGF-X7-treated animals, P<.01) and infarct transmurality in four out of five infarct scar segments (P<.05) on delayed contrast-enhanced MR imaging. Microscopic study confirmed the increase in capillary (P<.05) and arteriole (P<.05) density of infarct scar in pCK-HGF-X7-treated animals compared with pCK-LacZ control animals.
CONCLUSIONS: Hepatocyte growth factor gene (pCK-HGF-X7) delivered under MR guidance into infarct scar ameliorated global function and 3D strain, increased regional perfusion and infarct resorption, and enhanced angiogenesis/arteriogenesis. This feasibility study provides novel approach and analysis methods and instrumentation for delivering and evaluating new locally delivered therapies.
Full text links
Related Resources
Trending Papers
Proximal versus distal diuretics in congestive heart failure.Nephrology, Dialysis, Transplantation 2024 Februrary 30
World Health Organization and International Consensus Classification of eosinophilic disorders: 2024 update on diagnosis, risk stratification, and management.American Journal of Hematology 2024 March 30
Heart failure with preserved ejection fraction: diagnosis, risk assessment, and treatment.Clinical Research in Cardiology : Official Journal of the German Cardiac Society 2024 April 12
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app