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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site.
Chemical Communications : Chem Comm 2011 April 29
A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation through the formation of a β-hairpin around the ligation site.
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