[The -108 C/T polymorphism in paraoxonase 1 gene in Chinese patients with polycystic ovary syndrome].

OBJECTIVE: To investigate the relationship between the -108 C/T polymorphism in the promoter of paraoxonase 1 (PON1) gene and polycystic ovarian syndrome (PCOS) in Chinese women.

METHODS: A total of six hundred and sixty one subjects (346 patients with PCOS and 315 healthy control women) in Chengdu area were included in this study. The -108 C/T polymorphism in PON1 gene was studied using polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP). Total testosterone (TT), luteinizing hormone (LH), follicle stimulating hormone (FSH) and plasma insulin (INS) were determined by chemiluminescent method. Plasma glucose (GLU), total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were measured by enzymatic assay. Serum malondialdehyde (MDA) levels were measured by spectrophometry at 532 nm using micro-MDA detection kits.

RESULTS: The frequencies of CC, CT and TT genotypes at PON1 -108 C/T site in PCOS and control groups were 0.283 and 0.324, 0.517 and 0.492, 0.199 and 0.184, respectively, and the frequencies of C and T alleles in both groups were 0.542 and 0.570, 0.458 and 0.430, respectively. There were no significant differences in the frequencies of the genotypes and alleles between PCOS and control groups (P > 0.05). In the control group, the subjects with genotype CT had higher F-G scores and plasma glucose levels at 2 h during OGTT (75 g glucose) when compared with those of genotype CC (P < 0.05). Higher FSH level and MDA concentration also observed in those of genotype TT by compared with those of CC genotype (P < 0.05). The differences in FSH, GLU120 and MDA levels remained significant after adjustment for age and BMI (P = 0.006, 0.033 and 0.047, respectively). In the PCOS group, the patients with genotype CT had higher atherogenic index (AI) when compared with those of genotype CC (P = 0.038), but this difference was not significant after adjustment for age and BMI (P = 0.053).

CONCLUSION: This study indicated that the -108 C/T polymorphism in the promoter of PON1 gene was associated with hormonal levels, glucose metabolism and oxidative stress in the control women, but not associated with PCOS in Chinese women of Chengdu area.