Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage

Daowen Xiong, Tao Fang, Linpeng Yu, Xiaofeng Sima, Wentao Zhu
Science of the Total Environment 2011 March 15, 409 (8): 1444-52
The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO(2) NPs (96-h LC(50) of 124.5mg/L) to zebrafish was greater than that of the bulk TiO(2), which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn(2+) solution were quite similar to each other (96-h LC(50) of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs, ·OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of ·OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of ·OH. In contrast, exposure to 5mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50mg/L TiO(2) NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms.

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