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Usefulness of published PCR primers in detecting human rhinovirus infection.

Cassandra E Faux, Katherine E Arden, Stephen B Lambert, Michael D Nissen, Terry M Nolan, Anne B Chang, Theo P Sloots, Ian M Mackay
Emerging Infectious Diseases 2011 Februrary
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

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