JOURNAL ARTICLE

Backbone, side chain and heme resonance assignments of the triheme cytochrome PpcA from Geobacter sulfurreducens

Leonor Morgado, Vitor B Paix√£o, Carlos A Salgueiro, Marta Bruix
Biomolecular NMR Assignments 2011, 5 (1): 113-6
21069484
Gene knock-out studies on Geobacter sulfurreducens cells showed that the periplasmic triheme cytochrome PpcA is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI) oxides. The crucial role of this protein in bridging the electron transfer between the cytoplasm and cell exterior was further supported by proteomics studies. In comparison with non-heme proteins, the presence of numerous proton-containing groups in the heme groups causes additional challenges to the full protein assignment and structure calculation. Here, we report the complete assignment of the heme proton signals together with the (1)H and (15)N backbone and side chain assignments of the reduced form of PpcA.

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